Print this page

Facilities

Immunohistochemistry

The use of immunohistochemistry is recommended for differential diagnosis between neoplastic processes (e.g. B-cell lymphoma versus T-cell lymphoma, sarcoma versus carcinoma, neuroectodermal tumours, etc.), to establish prognostic factors in neoplastic processes, and to make a diagnosis of certainty in certain infectious diseases (coronavirus, herpesvirus, calicivirus, etc.).

Biological materials required:
- Tissue samples (excised formations, biopsies).
- Fluid samples - Intra-vitam diagnosis Feline Infectious Peritonitis.

Cell Markers

Identification

CD3

T- lymphocyte

CD79a

B lymphocyte/Plasma cell

CD18 (canin)

Macrophage/Histiocyte

CD11D (canin)

Macrophage (in spleen and bone marrow)

Desmin/Alpha-actine

Leiomyocyt/Rhabdomyocyt

Vimentin

Mesenchymal cells

Pan-Cytokeratin

Epithelial cells

CD117 (c-Kit)

Mast cells

Interstitial cells – cabaline (Cajal cells)

Factor VIII

Endothelial cells

CD45 R

B lymphocyte (feline)

S-100

Schwann cells/Melanocyte

Synaptophysin

Neuroendocrine cells/ganglionar cells

Neurofilaments

Neurons

Caspase 3

Apoptotic cells

Histiocytic antigen (mieloid)

Neutrophils, monocyte and young macrophages

PCNA

Cell growth factors

GFAP

Astrocite

Lysozyme

Macrophages

Pax-5

B lymphocytes

MHC II Ag

Major histocompatibility complex type II

Mast Cell Triptaze

Mast cell tryptase

Melan A

Melanocite

Albumin

Albumin

TNF-alpha (canine)

Tumoral necrosis factor

IL-6 (canine)

Interleukin 6

 

Infectious agent

Diagnosis

Feline coronavirus (FCoV)

Feline Infectious Peritonitis (FIP)

Feline Calicivirus (FCV)

Feline calicivirosis

Feline Herpesvirus (FHV)

Feline Herpesvirosis

Feline leukemia virus (FeLV)

Feline viral leukemia

Feline/Canine Parvovirus (FPV/CPV)

Feline/Canine Parvovirosis

Canine adenovirus

Infectious Canine Hepatititis (Rubarth disease)

Canine Distemper Virus (CDV)

Carre disease/ Canine Distemper Diseases

Toxoplasma gondii

Toxoplasmosis

Neospora caninum

Neosporosis

Helicobacter spp.

Helicobacter infection

 

Prognostic factors

Prognostic factors – oral melanoma/dermal canine (Ki-67 + COX-2 score)

Prognostic factors – canine mammary carcinoma/feline (COX-2 score)

Prognostic factors – canine osteosarcoma (COX-2 score)

Prognostic factors – Mast cell tumor (C-kit pattern + Ki-67 score)