Immunocytochemistry

The use of immunocytochemistry is recommended for differential diagnosis between neoplastic processes (e.g. B cell lymphoma VS T cell lymphoma, sarcoma VS carcinoma, etc.) and for diagnosis of certainty in certain infectious diseases (coronavirus).

Biological materials required:
- Cellularity collected by fine needle aspiration (FNA).
- Fluid samples - Intra-vitamin diagnosis Feline Infectious Peritonitis.

 

Cell Markers

Identification

CD3

T- lymphocyte

CD79a

B lymphocyte/Plasma cell

CD18 (canine)

Macrophage/Histiocyte

CD11D (canine)

Macrophage (in spleen and bone marrow)

Desmin/Alpha-actine

Leiomyocyt/Rhabdomyocyt

Vimentin

Mesenchymal cells

Pan-Cytokeratin

Epithelial cells

CD117 (c-Kit)

Mast cells

Interstitial cells – cabaline (Cajal cells)

Factor VIII

Endothelial cells

CD45 R

B lymphocyte (feline)

S-100

Schwann cells/Melanocyte

Synaptophysin

Neuroendocrine cells/ganglionar cells

Neurofilaments

Neurons

Caspase 3

Apoptotic cells

Histiocytic antigen (mieloid)

Neutrophils, monocyte and young macrophages

PCNA

Cell growth factors

GFAP

Astrocite

Lysozyme

Macrophages

Pax-5

B lymphocytes

MHC II Ag

Major histocompatibility complex type II

Mast Cell Triptaze

Mast cell tryptase

Melan A

Melanocite

Albumin

Albumin

TNF-alpha (canine)

Tumoral necrosis factor

IL-6 (canine)

Interleukin 6

 

Intra-vitam diagnosis

Viral entity

- dried slides
- fluid
Feline Infectious Peritonitis
(FIP)